Enzymes catalyze industrially important reactions in organic solvents. But their activity in anhydrous media is extremely low compared with its activity in water media. It is due to decrease of enzyme motion in the absence of lubricant water followed by enzyme flexibility decrease. Enzyme flexibility has relation to enzyme activity because enzyme does flexible motion for catalysis. Thus, enzyme flexibility modulation can lead enzyme activity enhancement. In this study, Candida antarctica lipase B(CalB) was selected as a model enzyme for mutations to change enzyme flexibility. From the analysis using spring model, it was detected that forced and twisted regions of CalB were located in residues surrounding active site. The edges of these helices were selected as target sites aiming at increasing flexibility. The details will be presented and discussed.
키워드
Enzyme activityCandida antarctica lipase BEnzyme flexibilityspring model
저자
So Yeon HONG [ Graduate Program in Bioengineering, Seoul National University,. ]
Young Je YOO [ Graduate Program in Bioengineering, School of Chemical and Biological Engineering, Seoul National University,. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동