Earticle

초록

영어

A novel enzyme support derived from cellulose and chitosan was prepared under an environment-friendly preparation processes using ionic liquids. Cellulose and chitosan were codissolved in [Emim] [acetate]. The obtained clear and viscous solution was introduced dropwise into water bath using a syringe needle. Then the hydrogel beads were collected and extensively washed with deionized water. Prepared cellulose-chitsoan composite bead was treated with glutaraldehyde and then used as the support to immobilize formate dehydrogenase. Formate dehydrogenase was successfully cross-linked on the surface of cellulose-chitosan bead. Immobilized enzyme was easily reused and remained activity after 5 times reuse was over 95% of initial activity. Thermostability and pH stability of formate dehydrogenase immobilized on cellulose-chitosan bead were also highly enhanced. Half-life times of immobilized enzymes were 1.8 and 7.1 times increased at 52℃ and pH 4.3, respectively.

키워드

저자

• Seulji AN [ Dept. of Microbial Engineering, Konkuk University, Seoul, 143-701. ]
• Min Hoo KIM [ Dept. of Microbial Engineering, Konkuk University, Seoul, 143-701. ]
• Young-Hoo KIM [ Dept. of Microbial Engineering, Konkuk University, Seoul, 143-701. ]
• Hyung Joo KIM [ Dept. of Microbial Engineering, Konkuk University, Seoul, 143-701. ]
• Keehoon WON [ Dept. of Chemical and Biochemical Engineeringm, Dongguk University, Seoul, 100-715. ]
• Sang Hyun LEE [ Dept. of Microbial Engineering, Konkuk University, Seoul, 143-701. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576