Se Jong HAN, Heeyong PARK, Sung Gu LEE, Hong Kum LEE, Joung Han YIM
언어
영어(ENG)
URL
https://www.earticle.net/Article/A119620
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
영어
The medium components were optimized by using a statistical experimental design approach for increasing chitinase production from the Antarctic bacterium Sanguibacter antarcticus KOPRI 21702. To increase the production of psychrophilic chitinase, significant carbon and nitrogen sources were determined and other elements were chosen by using a two-level Plackett-Burman statistical design technique. In the first optimization step, glycerol and peptone were selected as main carbon and nitrogen sources, respectively. In the second step, the influence of fifteen components included in Marine Broth medium on chitinase production was evaluated. As a result, NaCl, Fe(C6H5O7), MgCl2, Na2SO4, H3BO3, and CaCl2 were found to have a positive influence on the chitinase production, while KCl, KBr, and NH4NO3 had a negative influence. In the third step, the concentrations of NaCl, Fe(C6H5O7) and MgCl2 were further optimized. The maximum chitinase activity obtained was 89 U/L using 10 g/L glycerol, 5 g/L peptone, 23 g/L NaCl, 0.01 g/L Fe(C6H5O7), and 2.5 g/L MgCl2. [This work was supported by a grant from the Korea Polar Research Institute (PE10050).]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동