MITF (microphthalmia-associated transcription factor), a bHLH (basic helix-loop-helix) transcription factor with a leucine zipper, is a key regulatory protein for the pigmentation process in melanocytes. Binding of MITF to E-box caused transcription of several pigmenting genes including the tyrosinase gene. The purpose of this study is to analyze the interaction of MITF and its DNA binding site (E-box, CATGTG) by surface plasmon resonance. Label free DNA-protein interaction assay, SPR (surface plasmon resonance), coated by EDC (Ethyl-3-(3- dimethylaminopropyl)carbodiimide) / NHS (N-Hydroxysuccinimide) were used with amine coupling methods that permitted efficient immobilization of DNA on the gold surface. It is expected that SPR can be a useful method to study of MITF and E-box interactions.
키워드
Pigmentation processMITF and E-box interactionsSurface plasmon resonance
저자
Man-Ki SON [ Department of Biological Engineering, Inha University, Incheon, Korea. ]
Hyang-Bok LEE [ Department of Biological Engineering, Inha University, Incheon, Korea. ]
Dung HOANG NGUYEN [ Department of Biological Engineering, Inha University, Incheon, Korea. ]
SunA YOON [ Department of Biological Engineering, Inha University, Incheon, Korea. ]
Jimin UM [ Department of Biological Engineering, Inha University, Incheon, Korea. ]
Eun-Ki KIM [ Department of Biological Engineering, Inha University, Incheon, Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
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