Earticle

초록

영어

The ability to accurately determine intracellular concentrations of metabolites is of key importance in studying signaling and metabolic reaction networks and their regulation in vivo. In the last few years, much time has been invested in studying the intracellular metabolites concentration profiles of E. coli in the stationary phase. However, there are few studies related to the intracellular metabolites concentration profiles of E. coli in the exponential phase. In this study, metabolic regulation in E. coli was studied in terms of the change in intracellular metabolites (e.g., glucose-1-phosphate, fructose-1,6-bisphosphate, phosphoenolpyruvate, pyruvate, acetyl-coenzyme A, 6-phosphogluconate, ribulose-5-phosphate, xylulose-5-phosphate, erythrose-4-phosphate) concentration at two different growth phases, in batch
culture. Samples were taken every 2 h after batch cultivation started. Culture sample vials (5 mL) were plunged rapidly into an equal volume of 60% aqueous methanol (-40°C). The quenched biomass was centrifuged for 10 min at 3000×g and -9°C. An extraction procedure using 100% methanol (-48°C) was performed to extract intracellular metabolites from E. coli K12 strain W3110. All measurement were carried out on an HPLC system coupled with an ion trap mass pectrometer equipped with a turbo-ion spray source.

키워드

저자

• Changhun PARK [ Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 121-742, Republic of Korea. ]
• Changhun PARK [ Clinical Trials Center, Yonsei University Health System, Seoul 120-725, Republic of Korea. Department of Chemistry, Sogang University, Seoul 121-742, Republic of Korea. ]
• Han Bin OH [ Department of Chemistry, Sogang University, Seoul 121-742, Republic of Korea. ]
• Jinwon LEE [ Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 121-742, Republic of Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576