Earticle

초록

영어

The goal of this research is to achieve development of recombinant E. coli for fatty acid synthesis (FAS). The enzyme of aceE gene converts pyruvate to acetyl-CoA and that of accA gene catalyzes the addition of CO2 to acetyl-CoA to generate malonyl-CoA. The genes were identified as homologous gene of E. coli through a metabolic pathway. The genes for pyruvate dehydrogenase (aceE) and acetyl-CoA carboxylase (accA), which are the enzymes that catalyze the first step in the synthesis of fatty acids in Escherichia coli MG1655, were cloned. That is, the E. coli strains were midterm recombinant strains to produce fatty acid. The recombinant E. coli MG1655 containing the aceE or accA, and the two genes inserted expression vector (pTrc99A) were developed. To confirm effects of each gene, the intermediate products were analyzed from in vivo metabolites and in vitro metabolites of recombinant E. coli. As a
result, the three strains produced more malonic acid than its wild type E. coli. However, there was insignificant the difference between E. coli harboring a aceE gene-inserted pTrc99A vector and E. coli harboring a accA gene-inserted pTrc99A vector.

키워드

저자

• Eunyoung JEON [ Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 121-742. ]
• Sunhee LEE [ Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 121-742. ]
• Jinwon LEE [ Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 121-742. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576