Modified parylene film which contains aldehyde groups are applied as a linker layer of SPR biosensor. Parylene is a polymer of p-xylylene with good optical properties, chemical resistance against most organic solvents and relatively high mechanical strength which are required for the linker layer of SPR biosensor. In this work, parylene that was modified to have aldehyde groups was deposited on gold surface of the SPR biosensor as thin film. Under the film thickness of 50 nm, the SPR biosensor was correctly performed with no significant change of sensitivity. Trough covalent coupling between aldehyde groups and amine groups, proteins and peptides could be strongly immobilized on th surface of the parylene linker layer. To demonstrate improvement of immobilization efficiency, covalent immobilization of horseradish peroxidase (HRP) on the parylene linker layer was performed and SPR signal change from the immobilized HRP was compared with that from physically adsorbed HRP on gold surface of the SPR biosensor. The SPR biosensor with the modified parylene lanker layer was applied for diagnosis of rheumatoid arthritis. On the linker layer of the SPR biosensor, cyclic citrullinated peptide (CCP) was covalently immobilized and anti-CCP antibodies was detected from RA patient sera. The results from SPR were well corresponded to those from ELISA assay previously performed. Furthermore, regeneration of SPR biosensor was achieved by removal and re-deposition of the parylene linker layer by treating oxygen plasma
키워드
SPR biosensorparyleneCCP
저자
Hyuk KO [ School of Advanced Material Engineering, Yonsei University, 134 Shinchon-dong, Seodaemun-gu, Seoul, Korea. ]
Jo-Il KIM [ School of Advanced Material Engineering, Yonsei University, 134 Shinchon-dong, Seodaemun-gu, Seoul, Korea. ]
Moo-Hwan KIM [ Femto Science, 323-1, Banwol-dong, Hwaseong-si, Gyeonggi-do, Korea. ]
Jae-Chul PYUN [ School of Advanced Material Engineering, Yonsei University, 134 Shinchon-dong, Seodaemun-gu, Seoul, Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동