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Parylene film as a linker layer of SPR biosensor

첫 페이지 보기
  • 발행기관
    한국생물공학회 바로가기
  • 간행물
    한국생물공학회 학술대회 바로가기
  • 통권
    2010 춘계학술대회 및 국제심포지움 (2010.04)바로가기
  • 페이지
    pp.121-121
  • 저자
    Hyuk KO, Jo-Il KIM, Moo-Hwan KIM, Jae-Chul PYUN
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A119582

※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.

원문정보

초록

영어
Modified parylene film which contains aldehyde groups are applied as a linker layer of SPR biosensor. Parylene is a polymer of p-xylylene with good optical properties, chemical resistance against most organic solvents and relatively high mechanical strength which are required for the linker layer of SPR biosensor. In this work, parylene that was modified to have aldehyde groups
was deposited on gold surface of the SPR biosensor as thin film. Under the film thickness of 50 nm, the SPR biosensor was correctly performed with no significant change of sensitivity. Trough covalent coupling between aldehyde groups and amine groups, proteins and peptides could be strongly immobilized on th surface of the parylene linker layer. To demonstrate improvement of
immobilization efficiency, covalent immobilization of horseradish peroxidase (HRP) on the parylene linker layer was performed and SPR signal change from the immobilized HRP was compared with that from physically adsorbed HRP on gold surface of the SPR biosensor. The SPR biosensor with the modified parylene lanker layer was applied for diagnosis of rheumatoid arthritis. On the linker layer of the SPR biosensor, cyclic citrullinated peptide (CCP) was
covalently immobilized and anti-CCP antibodies was detected from RA patient sera. The results from SPR were well corresponded to those from ELISA assay previously performed. Furthermore, regeneration of SPR biosensor was achieved by removal and re-deposition of the parylene linker layer by treating oxygen plasma

키워드

SPR biosensor parylene CCP

저자

  • Hyuk KO [ School of Advanced Material Engineering, Yonsei University, 134 Shinchon-dong, Seodaemun-gu, Seoul, Korea. ]
  • Jo-Il KIM [ School of Advanced Material Engineering, Yonsei University, 134 Shinchon-dong, Seodaemun-gu, Seoul, Korea. ]
  • Moo-Hwan KIM [ Femto Science, 323-1, Banwol-dong, Hwaseong-si, Gyeonggi-do, Korea. ]
  • Jae-Chul PYUN [ School of Advanced Material Engineering, Yonsei University, 134 Shinchon-dong, Seodaemun-gu, Seoul, Korea. ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
  • 설립연도
    1984
  • 분야
    공학>생물공학
  • 소개
    이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다 1. 생물공학 분야의 발전을 위한 연구 협력 2. 생물공학의 실용화를 촉진시키기 위한 산학 협동 3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최 4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간 5. 생물공학 발전을 위한 정책 건의 6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동

간행물

  • 간행물명
    한국생물공학회 학술대회
  • 간기
    반년간
  • 수록기간
    1985~2013
  • 십진분류
    KDC 476 DDC 576

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