Sang Woo SEO, Jae-Seong YANG, Inhae KIM, Sanguk KIM, Gyoo Yeol JUNG
언어
영어(ENG)
URL
https://www.earticle.net/Article/A119554
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원문정보
초록
영어
"Metabolic Engineering" aims the purposeful redesign of the biological systems and requires the accurate information of the cellular metabolic networks and proper tools for the reconstruction of the biological. Numerous regulatory elements such as promoter libraries and RBS calculator can be applied for the modulation of gene expression. However, without carefully considering the structural information of 5’-unstranslated region (5’-UTR) sequence, it is insufficient to precisely design and modulate the gene expression level. To address this issue, in this study, we randomized 5’-UTRs maintaining ribosome binding affinity using superfolder GFP as a reporting system in Escherichia coli. A mathematical model was constructed by mapping between the secondary structures of 5’-UTRs and the expression level of superfolder GFP. Examples using the other genetic contexts will show the potentials of this model to predict the precise expression level based on the structural information and consequently will provide a valuable tool for "Metabolic Engineering".
Sang Woo SEO [ Department of Chemical Engineering, Pohang University of Science and Technology, San 31, Hyoja-dong, Nam-gu, Pohang, Gyeongbuk, Korea, 790-784. ]
Jae-Seong YANG [ School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, San 31, Hyoja-dong, Nam-gu, Pohang, Gyeongbuk, Korea, 790-784. ]
Inhae KIM [ Division of Molecular and Life Science, Pohang University of Science and Technology, San 31, Hyoja-dong, Nam-gu, Pohang, Gyeongbuk, Korea, 790-784. ]
Sanguk KIM [ School of Interdisciplinary Bioscience and Bioengineering and Division of Molecular and Life Science, Pohang University of Science and Technology, San 31, Hyoja-dong, Nam-gu, Pohang, Gyeongbuk, Korea, 790-784. ]
Gyoo Yeol JUNG [ Department of Chemical Engineering and School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, San 31, Hyoja-dong, Nam-gu, Pohang, Gyeongbuk, Korea, 790-784. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동