Earticle

초록

영어

Nattokinase is a potent fibrinolytic enzyme that is considered to be a promising agent for thrombosis therapy. The enzymes were discovered from various sources, such as Japanese natto, Korean Chungkookjang soy sauce, and Chinese douchi. Based on its food origin and relatively strong fibrinolytic activity, nattokinase has advantages over other commercially used medicine in preventative and prolonged effects, convenient oral administration, and stability in the gastrointestinal tract. In this study, Bacillus subtilis was cultivated to produce nattokinase.
Glucose and NH4Cl were used as carbon and nitrogen sources with peptone supplementation. In flask culture, peptone concentration was fixed at 50 g/L. The highest cell growth (optical density monitored at 600 nm) and nattokinase activity were obtained with 10 g/L of glucose supplementation. At this condition, nattokinase activity was 688 unit/ml. In fed-batch culture of B. subtilis in fermentor, the ratio of peptone and glucose in feeding solution was varied. The highest cell concentration and nattokinase activity were 75 g/L and 15,000 unit/ml, respectively.
Nattokinase activity increased with cell growth, showing growthassociated production.

키워드

저자

• Eun-Yeong KWON [ Department of Chemical Engineering, Chungbuk National University, Cheongju, Chungbuk 361-763. ]
• Beom Soo KIM [ Department of Chemical Engineering, Chungbuk National University, Cheongju, Chungbuk 361-763. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576