The diagnosis of infectious diseases is nowadays mostly performed by molecular methods that require multiplex detection and quantification of a wide range of pathogenic microorganisms. However, simultaneous multiplex quantitative detection of pathogens suffers from compromises between the level of multiplexing and accuracy of quantification. Here, we demonstrate the novel multiplex pathogen detection method using capillary electrophoresis-single strand conformation polymorphism (CE-SSCP) coupled with multiplex ligation-dependent probe amplification (MLPA). MLPA-CE-SSCP is composed of four major steps which are probe hybridization, probe ligation, multiplex amplification and detection. Using ten foodborne pathogens as a model set, all the steps were carefully optimized to precisely quantify by MLPA-CE-SSCP. We could obtain the results which illustrate a strong potential in clinical diagnosis, food safety, and biosafety.
키워드
CE-SSCPMLPAPathogen detection
저자
Boram CHUNG [ I-Bio program, Postech, Pohang. ]
Won Shin GI [ I-Bio program, Postech, Pohang. ]
Gyoo Yeol JUNG [ I-Bio program and Dept. of Chemical Engineering, Postech, Pohang. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동