Earticle

초록

영어

Recent studies uncover that RNA does much more than a passive carrier of genetic information. For example, RNA shows an increasing range of cellular functionality including selective binding affinity toward target ligands and regulation of gene expression through a direct sensing of
metabolites. The versatile functions of RNA in living cells have enabled the natural RNA devices to be engineered into synthetic riboswitches, permitting programmable genetic responses directly from chemical cues without the aid of protein receptors. Here we present synthetic
riboregulatory systems to control prokaryotic and eukaryotic gene expressions by small molecules. The riboregulatory circuits are designed by incorporation of aptamer and ribozyme sequences in 5’-UTR or 5’-proximal coding region of a reporter gene. Sequence design of the riboswitch is easily achieved to fine-tune mRNA secondary structures and the consequent switching behaviors including target specificity and sensitivity. Owing to the good sequence designability, the riboswitch seems promising for biomedical applications to high-throughput
screening systems, biosensor and in vivo bioimaging.

저자

• Jeongtae SON [ Department of Biotechnology, Yonsei University, Shinchon-Dong 134, Seodaemun-Gu, Seoul 120-749, South Korea. ]
• Dong-Joon KO [ Department of Biotechnology, Yonsei University, Shinchon-Dong 134, Seodaemun-Gu, Seoul 120-749, South Korea. ]
• Jong-Shik SHIN [ Department of Biotechnology, Yonsei University, Shinchon-Dong 134, Seodaemun-Gu, Seoul 120-749, South Korea. ]

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간행물

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• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576