In this presentation, we report a quick and reproducible surface-enhanced Raman scattering (SERS)-based immunoassay technique, using hollow gold nanospheres (HGNs) and magnetic beads. HGNs show strong SERS enhancement effects from individual particles because hot spots can be localized within pinholes in the hollow particle structure. Accordingly, HGNs can be used for highly reproducible immunoanalysis of cancer markers. Magnetic beads were used as supporting substrates for the formation of the immunocomplex. In addition, we have implemented this SERS-based immunoassay, performed in a microtube, into a microfluidic platform. The SERSbased optofluidic sensor is composed of three compartments consisting of the gradient channel that serially dilutes the target marker, the injection and mixing area of antibody-conjugated hollow gold nanospheres and magnetic beads and the trapping area of sandwich immunocomplexes using multiple solenoids. Quantitative analysis of a specific target marker is performed by analyzing its characteristic SERS signals. For validation, a well-known lung cancer marker, carcinoembryonic antigen (CEA), was used as a target. Based on experimental results, the limit of detection (LOD) was determined to be 1–10 pg/mL, this value being about 100–1000 times more sensitive than the LOD of conventional enzyme-linked immunosorbent assay (ELISA). Our proposed SERS-based optofluidic sensing technology has many advantages over previously reported SERS detection methods, such as good reproducibility, low limits of detection and fast assay times. Accordingly, this technique is expected to be a powerful clinical tool for fast and reliable cancer diagnosis.
저자
Jaebum CHOO [ Department of Bionano Engineering, Hanyang University, Ansan, KOREA. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동