To investigate antioxidant activity of the culture broth from submerged culture of Tremella fuciformis, we preferentially analyzed the chemical composition of culture broth, which was mainly composed of carbohydrate (296.39 mg/g) and protein (9.24 mg/g), respectively. Also, contents of polyphenols, flavonoids and flavonols were 16.63 mg/g, 9.19 mg/g and 83.74 μg/g, respectively. Next, we examined the scavenging abilities on DPPH (1,1-diphenyl-2-picrylhydrazyl) radical, ABTS (2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) radical, the reducing power, and chelating ability on ferrous ions. All antioxidant activities of the culture broth were increased in proportion to its concentrations. The IC50 values were in order as follows ABTS radical scavenging activity < DPPH radical scavenging activity < chelating power. Accordingly, these results suggest that pharmacological function of T. fuciformis might be due to, at least partially, their protective effects against oxidation and the culture broth of T. fuciformis was free radical inhibitors or scavengers at low concentration, involving possibly in termination of free radical reaction as primary antioxidants.
목차
Abstract Introduction1 MATERIALS AND METHODS Chemicals Cultivation of T. fuciformis and Sample Preparation Analysis of Chemical Components DPPH Radical Scavenging Activity ABTS Radical Scavenging Activity Measurement of Reducing Power Assay of Ferrous ion Chelating activity RESULTS AND DISCUSSION Chemical Composition of Culture Broth DPPH Radical Scavenging Activity ABTS Radical Scavenging Activity Reducing Power Fe2+ Chelating Activity IC50 Value in Antioxidant Properties Acknowledgements REFERENCES
키워드
Tremella fuciformisantioxidantscavenging activityreducing power
저자
나경수 [ Kyung Soo Ra | 대구공업대학 식품영양과 ]
최장원 [ Jang Won Choi | 대구대학교 바이오산업학과 ]
Corresponding author
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
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