Lipozyme TLIM을 효소적 반응촉매로 이용한 glyceryl monooleate로부터의 diacylglycerol의 합성
Production of Diacylglycerol from Lipase by the Catalyzed Reaction of Soybean Oil and Glyceryl Monooleate
Diacylglycerol (DAG) was produced from lipase by the catalyzed synthesis of soybean oil (SBO) and glyceryl monooleate (GMO) with Lipozyme TLIM (Thermomyces lanuginosa). Effects of reaction time, molar ratio and enzyme road were studied. When 2:1, 1:1 and 1:2 (SBO:GMO) molar ratios with 20% Lipozyme TLIM were applied in a 1-hr reaction, the concentrations of DAG produced were 17.8, 20.0 and 20.4 g/100 g oil, respectively. Different amounts (2, 5, 10 and 20%) of Lipozyme TLIM were used at a 1:2 (SBO:GMO) molar ratio, and the concentrations of DAG produced in a 1-hr reaction were 10.8, 14.0, 16.9 and 20.4 g/100 g oil, respectively. During a 72-hr reaction, 10.8-22.7 g/100 g oil of DAG were produced under the reaction conditions in this study.
목차
Abstract 서론 재료 및 방법 재료 DAG의 효소적 합성 Normal phase HPLC 분석 및 정량 결과 및 고찰 DAG의 합성과 NP-HPLC 분석 및 정량 요약 문헌