면역활성 증진을 위한 수삼의 상황버섯 균사체 고체배양으로 조제된 수삼발효물로부터 활성 다당류 분획
Enhancement of Immunostimulation by Fractionation of Active Polysaccharide from Fermented Ginseng with Phellinus linteus Mycelium in Solid Culture
Crude polysaccharide (WG-PL-CP) was fractionated from fermented ginseng with Phellinus linteus in solid culture to enhance the immunostimulation of ginseng. WG-PL-CP produce three active polysaccharide-rich fractions (WGPL- CP-II, III, and IV) on DEAE-Sepharose CL-6B (Cl− form). WG-PL-CP-III displayed higher mitogenic activity (1.98- fold of the saline control at 100 μg/mL) than did WG-CP-III or PL-CP-III (1.60- or 1.65-fold, respectively), and potent intestinal immune system modulating activity through Peyer's patch was obtained by WG-PL-CP-IV only (1.56-fold). Meanwhile, WG-PL-CP-II and III significantly enhanced macrophage stimulating activity (2.01- and 1.94-fold) compared to WG-CP-II and III (1.73- and 1.66-fold) or PL-CP-II and III (1.79- and 1.72-fold). In addition, WG-PL-CP-III and IV mainly contained neutral sugar (73.5 and 67.3%) and uronic acid (23.2 and 24.6%). Component sugar analysis also showed that WG-PL-CP-III consisted mainly of uronic acid as well as the neutral sugars Glc, Ara, Gal, Rha and Xyl (molar ratio of 0.81:1.00:0.49:0.42:0.28:0.20), whereas WG-PL-CP-IV was mainly comprised of uronic acid, Ara, Rha, Gal, Xyl and Glc (1.00:0.75:0.69:0.63:0.42:0.34). Therefore, it is assumed that these active polysaccharides play an important role in enhancing the immunostimulation of fermented ginseng with P. linteus in solid culture.
목차
Abstract 서론 재료 및 방법 수삼의 상황버섯 균사체 고체배양을 통한 발효물 조제 수삼-상황버섯 균사체 발효물로부터 열수추출물과 조다당획분조제 수삼-상황버섯 균사체 발효물 조다당으로부터 면역활성 다당획분의 분획 면역활성 다당획분의 성분 및 구성당 분석 실험동물과 시약 비장세포의 마이토젠 활성 Peyer’s patch를 경유한 장관면역 활성 마크로파지 활성 통계처리 결과 및 고찰 수삼-상황버섯 균사체 발효물의 열수추출물 및 다당체의 면역활성 수삼-상황버섯 균사체 발효물로부터 얻은 다당체 분획에 따른면역활성 수삼-상황버섯 균사체 발효물로부터 얻은 면역활성 다당체 획분의 특성 요약 문헌