Earticle

초록

영어

Rosa rugosa has traditionally been used as a folk remedy for diabetes. The objective of this study was therefore to demonstrate the inhibition of endothelial dysfunction activities through antioxidants and the anti-glycation of Rosa rugosa roots. Dried roots of Rosa rugosa were boiled in methanol for three hours, evaporated and lyophilized with a freeze-dryer. The methanolic extract of Rosa rugosa roots (RRE) was tested for antioxidant activities by measuring total polyphenol (TP) content, flavonoid content, 1,1-diphenyl-2-picrylhydrazyl free radical-scavenging activity (DPPH) assay, and ferric-reducing antioxidant power (FRAP) assay. The total TP content, flavonoid content, FRAP value, and DPPHSC 50 are 345.2 μg gallic acid equivalents/mg dry matter (DM), 128.1 μg quercetin equivalents/mg DM, 2.2 mM FeSO4/mg DM and 34.2 μg DM/mL, respectively. Treatment of RRE significantly lowered fluorescent formation due to advanced glycation reaction. In addition, reactive oxygen species (ROS) scavenging assay, monocyte adherent assay and transendothelial electrical resistance (TEER) assay were performed to investigate the possibility that RRE improves endothelial dysfunction-induced diabetic complications. The adhesion of THP-1 to treated HUVEC with RRE (100 μg/mL; 33% and 500 μg/mL; 75%) was significantly reduced compared to HUVEC stimulated by glyceraldehydes-AGEs (advanced glycation end product). The TEER value (88 Ω·cm2) of stimulated HUVEC by glyceraldehydes-AGEs was reduced compared to non-stimulation (113 Ω·cm2). However, normalization with RRE increased endothelial permeability in a dose-dependent manner (100 μg/mL; 102 Ω·cm2 and 500 μg/mL; 106 Ω·cm2). Thus, these results suggest that Rosa rugosa roots could be a novel candidate for the prevention of diabetic complications through antioxidants and inhibition of advanced glycation end product formation.

목차

Abstract
 서론
 재료 및 방법
  재료
  해당근 추출물의 제조 및 주요 성분 분석
  항산화력 측정
  최종당화산물의 제조 및 확인
  추출물의 세포 독성 확인
  활성산소종(reactive oxygen species, ROS) 소거능 측정
  세포 배양 및 단핵구 부착
  Transendothelial electrical resistance(TEER) 값 측정
  통계 처리
 결과 및 고찰
  항산화력 측정
  활성산소종(reactive oxygen species, ROS) 소거능 측정
  최종당화산물의 제조 및 확인
  세포 독성 확인
  세포 배양 및 단핵구 부착
  TEER 값 측정
 요약
 문헌

키워드

저자

• 남미현 [ Mi-Hyun Nam | 고려대학교 생명공학부 ]
• 이현순 [ Hyun-Sun Lee | 고려대학교 보건과학연구소 ]
• 홍충의 [ Chung-Oui Hong | 고려대학교 생명공학부 ]
• 구윤창 [ Yoon-Chang Koo | 고려대학교 생명공학부 ]
• 서문영 [ Young Seomun | (주)바이오버드 ]
• 이광원 [ Kwang-Won Lee | 고려대학교 생명공학부 ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국식품과학회지 [Korean Journal of Food Science and Technology]

• 간기

  격월간

• pISSN

  0367-6293

• 수록기간

  1969~2011

• 십진분류

  KDC 574 DDC 664