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Agrobacterium tumefaciens-Mediated Genetic Transformation: Mechanism and Factors

첫 페이지 보기
  • 발행기관
    강원대학교 산림과학연구소 바로가기
  • 간행물
    Journal of Forest and Environmental Science 바로가기
  • 통권
    제25권 제3호 (2009.12)바로가기
  • 페이지
    pp.195-204
  • 저자
    Nitish Kumar, K.G. Vijayanand, Muppala P. Reddy, Amritpal S. Singh, Subhash Naraynan
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A118499

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초록

영어
Agrobacterium–mediated genetic transformation has been widely used for the production of genetically modified transgenic plants to obtain specific desired traits. Most of the molecular mechanisms that underlie the transformation steps have been well elucidated over the years. However, a few steps, such as nuclear targeting, T-DNA integration, and Agrobacterium-plant proteins involved remain largely obscure and are still under extensive studies. This review describes the major steps involved in the molecular mechanism of Agrobacterium-mediated transformation and provides insight in the recent developments in studies on the Agrobacterium-mediated genetic transformation system. Some factors affecting the transformation efficiency are also briefly discussed.

목차

ABSTRACT
 INTRODUCTION
 Agrobacterium-mediated gene transfer
 Mechanism of Agrobacterium infection,T-DNA transfer and integration
 Factors affecting Agrobacterium infectionand transformation efficiency
  Bacterial strain / vector
  Pre-culture/wounding
  Bacterial density and growth phase
  Inducers of vir genes
  Plant variety and explant
 Conclusion and perspective
 References

저자

  • Nitish Kumar [ Department of Agricultural Biotechnology, Anand Agricultural University ] Corresponding author
  • K.G. Vijayanand [ Discipline of wasteland research, Central Salt & Marine Chemicals Research Institute ]
  • Muppala P. Reddy [ Plant Stress Genomics and Technology Center, King Abdullah University of Science and Technology ]
  • Amritpal S. Singh [ Department of Agricultural Biotechnology, Anand Agricultural University ]
  • Subhash Naraynan [ Department of Agricultural Biotechnology, Anand Agricultural University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    강원대학교 산림과학연구소 [Institute of Forest Science Kangwon National University]
  • 설립연도
    1975
  • 분야
    농수해양>임학
  • 소개
    강원대학교부설산림과학연구소(이하 “연구소”라 한다)는 산림에 관한 제반 학술적 연구를 통하여 산림자원의 효용을 밝히고 임업 및 임산업의 발전에 기여함을 목적으로 한다.

간행물

  • 간행물명
    Journal of Forest and Environmental Science [산림과학연구]
  • 간기
    계간
  • pISSN
    2288-9744
  • eISSN
    2288-9752
  • 수록기간
    1981~2026
  • 등재여부
    KCI 등재
  • 십진분류
    KDC 526 DDC 634

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