Earticle

초록

영어

Proteins have wide applications in the medical, industrial and agricultural fields. They are usually produced in huge quantities in host cells such as Escherichia coli. However, high-level expression of recombinant proteins in E. coli often results in accumulation of insoluble aggregates known as inclusion bodies (IBs), thus requiring further solubilization, refolding and purification procedures to achieve functionally active products. Molecular chaperones have been applied successfully to refold various proteins both in vivo and in vitro, opening a new era in protein refolding. However, the exact function of individual molecular chaperones and the interaction between target protein and molecular chaperones are still unclear. We demonstrated
that refolding cocktail comprising ClpB/DnaKJE with or without the presence of ATP regeneration system and a refolding additive, PEG, could significantly enhance the refolding efficiency of heat-denatured malate dehyrogenase (MDH). To further clarify the individual or synergistic roles of each chaperone, various molecular chaperones including His-ClpB, His-DnaK, His-DnaJ, His-GrpE, His-Gro EL, His- Gro ES, and His-Trigger Factor were recently cloned, expressed and purified through IMAC, respectively. Currently, chaperoning efficiencies of these His-tagged
molecular chaperones are being studied systematically using heat- or chemicaldenatured
model proteins of different aggregate size. It is expected that our study will provide a better understanding of chaperone-assisted refolding process, thereby facilitating engineering implementation of a novel refolding strategy, folding-likerefolding approach based on refolding cocktail.

키워드

저자

• Lin ZHAI [ School of Chemical Engineering, Sungkyunkwan University ]
• Jiryang JANG [ School of Chemical Engineering, Sungkyunkwan University, ]
• Sung-eun KIM [ School of Chemical Engineering, Sungkyunkwan University, ]
• Rui NIAN [ School of Chemical Engineering, Sungkyunkwan University, ]
• Woo Seok CHOE [ School of Chemical Engineering, Sungkyunkwan University, ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576