Humans have different characteristics such as skin colors, physical constitutions, and disease incidences. Most of them are caused by single nucleotide polymorphism (SNP), a DNA sequence variation occurring when a single nucleotide in the genome differs between members of species. For multiple SNP analysis, we studied a technique named single base extension-end labeled free solution electrophoresis (SBE-ELFSE). ELFSE needs drag-tags that give uniform drag force to target DNAs in free solution. General requirements for the optimum drag-tags are high water-solubility, monodispersity, and charge neutrality. To fulfill these requirements, we designed and produced repetitive polypeptide drag-tags. We designed repetitive polypeptides sequenced as [GAGASGAGATGAGASGAGAT]n and expressed them with protein expression vector, pET32a. We are attempting SNP analysis using these repetitive polypeptides conjugated with DNAs which have different sizes. We expect that fast and accurate SNP analysis is accomplished based on our study.
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
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