SeungJin JUNG, ShengDe JIN, Sang-Il YUN, Doman KIM
언어
영어(ENG)
URL
https://www.earticle.net/Article/A115356
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
영어
Streptococcus pneumoniae is a major cause of invasive infection in young infants and older adults. Capsular polysaccharide (CPS) produced by S. pneumoniae is a virulence factor. CPS has been used as a vaccine. Purification of CPS is complicate process and requires many kinds of reagents and column chromatography method. In this study, purification process of CPS from S. pneumoniae serotype 19A was simplified by ethanol fractionation and pH adjustment as following: First the cultured broth was removed a precipitant by ethanol fractionation (50~80%, v/v). The precipitant with less than 50% and more than 80% of ethanol contained most of the contaminated proteins. Precipitations in both ethanols 50% and 80% were performed at neutral pH (7.0) by NaOH adjustment. Small carbohydrates and various contaminated cell hydrolyzates were easily removed by dialysis with dH2O.
키워드
Capsular polysaccharidepurificationethanol
저자
SeungJin JUNG [ Interdisciplinary Program of Graduate School for Bioenergy & Biomaterials, Chonnam National University, Gwangju 500-757, Korea. ]
ShengDe JIN [ Department of Biological Science and Technology, School of Biological Sciences and Technology, Chonnam National University, Gwangju 500-757, Korea. ]
Sang-Il YUN [ Department of Biological Science and Technology, School of Biological Sciences and Technology, Chonnam National University, Gwangju 500-757, Korea. ]
Doman KIM [ Department of Biological Science and Technology, School of Biological Sciences and Technology, Chonnam National University, Gwangju 500-757, Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동