The DNA of analyses are important in many area of research. Extensive theoretical investigations for DNA focus on the base pairs guanine and cytosine in nucleotide. Using method for analysis of guanine and cytosine were HPLC (High Performance Liquid Chromatography) and their chromatograms simulated by Aspen chromatography. C18 HPLC column and water/methanol/acetic acid mixture (90/10/0.2) were applied to separation guanine and cytosine. Chromatographic parameters (selectivity, resolution and number of theoretical plate) were calculated under different flow rates and sample concentrations. Calculated numbers of theoretical plate, retention time and porosity were utilized for simulation by Aspen chromatography, and we compared the simulations with experimental data.
키워드
guaninecytosineAspen chromatography
저자
Moon Bae PARK [ Dept. of Chemical Engineering, Chungnam National University ]
Jong Kyung PARK [ Dept. of Chemical Engineering, Chungnam National University ]
In Ho KIM [ Dept. of Chemical Engineering, Chungnam National University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
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