Seon-hwa SEO, Min-seon SONG, Shuwen WANG, Woo-seok CHOE
언어
영어(ENG)
URL
https://www.earticle.net/Article/A115346
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원문정보
초록
영어
Silica-coated magnetite (Fe3O4) nanoparticles were surface-modified with iminodiacetic acid (IDA) to enable metal ion chelation1. The IDA-functionalized magnetite particles of various sizes ranging from 200 to 350 nm were produced by controlling two factors (i.e. sonication time and substrate feed rate) during the modification of nanoparticles. It is believed that sonication time is likely to affect aggregation of particles and the feed rate of tetraethyl orthosilicate(TEOS) control the formation of polysiloxane on the surface. Cu2+-charged IDA2 superparamagnetic nanoparticles of various diameters were used to investigate the size-dependent protein adsorption behavior of particles using bovine serum albumin as a model protein. It was found that the size of nanoparticles is closely related to binding affinity and capacity of the adsorbent since higher maximum adsorption capacity (Qm) and lower binding affinity (i.e. larger dissociation constant (Kd)) are exhibited with the increase in particle size. This size-dependent differential protein binding behavior Cu2+-charged IDA nanoparticles will be conducive to designing nanoparticle adsorbents of tailored affinity and binding capacity most opted for target protein purification or impurity removal applications.
키워드
Magnetic nanoparticlesIDABSA adsorptionSizedependent
저자
Seon-hwa SEO [ School of Chemical Engineering, Sungkyunkwan University ]
Min-seon SONG [ School of Chemical Engineering, Sungkyunkwan University ]
Shuwen WANG [ School of Chemical Engineering, Sungkyunkwan University ]
Woo-seok CHOE [ School of Chemical Engineering, Sungkyunkwan University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동