Earticle

초록

영어

N-glycosylation status of purified b-haptoglobin separated from sera of patients with prostate cancer was studied in comparison to that of sera from patients with benign prostate diseases, or normal subjects. Two different approaches, as summarized below, one based on binding of lectins and antibodies to b-haptoglobin, the other on mass spectrometry of released N-linked glycans from b-haptoglobin, were performed. Some of the results were useful for distinction of prostate cancer vs. benign prostate diseases. (i) Binding of Phaseolus vulgaris-L lectin (PHA-L), defining the GlcNAcb6Mana6Man side chain present in tri- or tetra-antennary N-linked glycans, to b-haptoglobin was higher for cases of prostate cancer and high-grade prostate intraepithelial neoplasia than for benign diseases. Binding of Aleuria aurantia lectin (AAL) defining a3-, a4-, or a6FucGlcNAc, or monoclonal antibody directed to sialyl-Lex, to b-haptoglobin was also higher for some of the cancer cases than for benign diseases. Many other lectins and antibodies showed no binding to b-haptoglobin, or showed no significant difference between cancer vs. benign diseases. (ii) Mass spectrometric analysis of N-linked glycans of b-haptoglobin released by Peptide N-glycosidase-F showed enhanced expression of monosialyl tri-antennary structures in prostate cancer cases. Thus, binding of PHA-L to affinity-purified b-haptoglobin from sera of patients could lead to development of useful tools for differential diagnosis of prostate cancer vs. benign prostate diseases.

키워드

저자

• Seung-Yeol Park [ Division of Biomembrane Research, Pacific Northwest Research Institute, and Departments of Pathobiology and Global Health, University of Washington, Seattle, WA 98122, USA. 2Department of Biological Sciences, Korea Advanced Institute of Science and Technology ]
• Seon-Joo Yoon [ Division of Biomembrane Research, Pacific Northwest Research Institute, and Departments of Pathobiology and Global Health, University of Washington, Seattle, WA 98122, USA. ]
• Poh-Choo Pang [ Division of Molecular Biosciences, Faculty of Natural Sciences, Imperial College London, London SW7 2AZ, UK. ]
• Jenni Gallagher [ Seattle Urological Associates, 1221 Madison, Seattle, WA 98104, USA ]
• James E. Gottesman [ Seattle Urological Associates, 1221 Madison, Seattle, WA 98104, USA ]
• Anne Dell [ Division of Molecular Biosciences, Faculty of Natural Sciences, Imperial College London, London SW7 2AZ, UK. ]
• Jung-Hoe Kim [ Department of Biological Sciences, Korea Advanced Institute of Science and Technology ]
• Sen-itiroh Hakomori [ Division of Biomembrane Research, Pacific Northwest Research Institute, and Departments of Pathobiology and Global Health, University of Washington, Seattle, WA 98122, USA. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576