Based on previous in vitro studies showing that stimulation with glutamate could increase the number of cells expressing tyrosine hydroxylase (TH) protein, we investigated whether glutamatergic mechanisms also regulate induction of the dopaminergic neuronal phenotype by treating primary cultures obtained from transgenic TH/lacZ mice and monitoring the number of β-gal-immunolabeled cells. Primary cultures were stimulated after 1 day in vitro (DIV) with glutamic acid (0.1-1 μM) added in pulses followed by a washout to prevent desensitization. Glutamic acid induced the expression of the representative dopaminergic neuronal marker TH and the second enzyme for the biosynthesis of dopamine, AADC, at a moderate level. Of interest, the expression of GTPCH, an enzyme for the biosynthesis of dopamine, was increased twofold by glutamic acid. The induction of those neuronal markers was blocked by pretreatment with kyurenic acid (100 μM), a broad spectrum excitatory amino acid antagonist. These results suggest that glutamic acid regulates dopaminergic neuronal markers in olfactory bulb cells.
키워드
Glutamic acidOBneuronal markers
저자
Hong Sung CHUN [ Dept. of Biotechnology, Chosun University, Gwangju, 501-759, Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
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2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
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