Ultraviolet (UV) A radiation is one of the most important environmental factors involved in the skin aging 1). However, the expression pattern of skin-aging related proteins and skin-aging mechanism in UVA irradiated-fibroblasts are yet to be investigated. Skin-aging mechanism of induced-fibroblast by UVA radiation was studied using proteome analysis. The fibroblasts were cultured in Dulbecco's Modified Eagle's Medium (DMEM) with or without UVA irradiation. The proteome of cultured human fibroblasts were profiled using 2D-PAGE 2). Isoelectric focusing was carried out using commercial immobilized dry strips 3-10, 24cm. SDS-PAGE was performed using 25 x 21cm, 12 % polyacrylamide gel. Gels were stained with silver nitrate, analyzed by image master 2D platinum 7. In conclusion, we could identify that many proteins affect the skin aging, were up or down-regulated by UVA irradiation.
키워드
UVAskin agingproteome
저자
Mi Ae YOO [ Cosmetic Science Major, Applied Biotechnology Program, Graduate School, Ajou University ]
Sang Yo BYUN [ Cosmetic Science Major, Applied Biotechnology Program, Graduate School, Ajou University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동