Development of Optimal Production Medium with Cassava as a Carbon Source for Production of Functional Exopolysaccharides (EPS), a Cosmetic Biopolymer Using Improved Strains of Schizophyllum commune.
Recently all the nations are competitively focusing on developing biorefinery technologies. Biorefinery is defined as a bioprocess to produce high-valued industrial chemicals and energy using natural biomasses which are present enormously in nature. Cassava is a good alternative biomass source which could be used to produce various biorefinery products. The roots of cassava are mainly composed of 20∼25% starches and contain plenty of valuable nutrients such as vitamin C and calcium. Recently, it has been reported that a newly-developed super cassavas is being tested to be used as a carbon and energy source in biorefinery industries. Our research team intended to develop an economic bioprocess for mass production of exopolysaccharides(EPS) using cassava as a carbon source in liquid mycelial fermentations of Schizophyllum commune [1]. This product has the potent capability of maintaining high moisturized conditions on epidermal tissue, and also having positive effects on the restoration of epidermal tissue. First of all, medium optimization process was carried out in order to find an optimal composition of the production medium containing the cassava biomass. Approximately 2~3 fold increase in EPS productivity was observed in the shake flask cultures performed with the optimized production medium [2]. For the purpose of enhancing the EPS production, intensive strain improvement program was also carried out by obtaining large amounts of protoplasts for the isolation of single colonies. Mass screening for high-yielding producers was possible through the formation of the protoplasts of S. commune because high concentration of protoplasts(5x106 protoplasts/ml) could be recovered with efficient cell wall disrupting enzymes. Notably, the protoplast regeneration rate was as high as 98% when RPA medium containing 1.2M sorbitol was used as a regeneration medium.
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
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