Citron (Citrus junos Sieb ex TANAKA) is a citrus fruit that is cultivated in northeast Asia. Citron is known for containing abundant antioxidants such as vitamin C, flavonoids, for example hesperidin and hesperetin, and terpenoids such as limononin. When mature citron is processed for tea or other beverage food products in Korea, massive amounts of seeds and pericarp are remained as waste. This study was aimed to exploit the processed remnant of Citron for developing functional cosmetic applications. Analyses of extracts of seeds and pericarp identified spots that have the same Rf values of limonin and hesperidin respetively analyzed by TLC. The two extracts did not show significant radical scavenging activities measured by DPPH(2,2-diphenyl-1- picrylhydrazyl) method. But citron extracts have much high content of phenolic compounds. Cultured human dermal fibroblasts and HaCaT keratinocytes were irradiated with 25mJ UVB and the citron extracts were added to the medium of each culture. Cellular damages caused by UVB irradiation were prevented by the addition of the citron extract. In addition, the reduction of the enhanced MMP1(Matrix Metalloproteinase) expression after irradiation of UVB in human dermal fibroblasts was observed. Also the increased level of of pro-inflammtory TNF-α in the UVB irradiated HaCaT cells were decreased. Further analyses of the effect of citron extracts on collagen production and the expression of other pro-inflammatory cytokines will be performed. In conclusion, citron extract seems to have anti-photoaging effect and are expected to be used as a potential anti-aging agent.
키워드
Citronanti-agingMMPsanti-inflammatory
저자
Dasel KIM [ Department of Chemical and Biochemical Engineering, Dongguk University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동