Earticle

초록

영어

Odorant perception begins from the specific interaction with olfactory receptors and odorants. Olfactory receptors, belonging to G-protein coupled receptor family, are integral membrane proteins composed of seven transmembrane spanning domains. The protein-based olfactory bioseonsors using olfactory receptors might be good tools for odorant detection. For developing protein-based olfactory sensor with high selectivity and sensitivity, olfactory receptor proteins were expressed in E. coli. Due to their complicated form, these proteins are hardly expressed in the inner membrane in E. coli and are majorly expressed as inclusion body form. Previous studies shows that the inclusion body form of olfactory receptor has function for odorant detection when it was applied to various sensing devices such as carbon nanotube, conducting nanopolymer and QCM. In this study, the olfactory receptor proteins OR520, which specifically binds to helional, are purified from E. coli using detergent and reconstituted into liposome to have more functional form. Odorant detection assay carried out using surface plasmon resonance (SPR). The inclusion body form and reconstituted form of the proteins are immobilized on the gold surface of SPR chip and the results of odorant detection were compared.

키워드

저자

• Eun Hae OH [ School of Chemical and Biological Engineering, Seoul National University, Seoul, Korea. ]
• Sang Hoon LEE [ School of Chemical and Biological Engineering, Seoul National University, Seoul, Korea. ]
• Hyun Seok SONG [ School of Chemical and Biological Engineering, Seoul National University, Seoul, Korea. ]
• Joung Hyun LIM [ School of Chemical and Biological Engineering, Seoul National University, Seoul, Korea. ]
• Tai Hyun PARK [ School of Chemical and Biological Engineering, Seoul National University, Seoul, Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576