Earticle

초록

영어

It is important to monitor or measure toxicity of environmental pollutants or new drugs. Cell chip technology has become a promising tool because high-throughput and sensitive screening is possible. In the present work, we propose "gluing" method for cell chip construction by
displaying mussel adhesive proteins (MAPs) on the surface of target cells (insect Drosophila S2 and mammalian Chinese hamster ovary (CHO) DG44). Mussels use their adhesive proteins, fp-5, in order to attach on the rock in the tough sea. In the present work, we present construction of recombinant baculovirus expression vector, rBV-FP5, infection of recombinant viruses to target cells for expression and location of MAP on cell surface, and construction of cell chip using
MAP-displayed infected cells. Our proposed cell chip construction method does not need any harmful chemical linkers for cell immobilization. In addition, we would construct universal recombinant baculovirus expression system which can give adhesion ability to target cells. The
constructed cell chip system is applicable for environmental toxicity sensor or drug screening at a cellular level.

키워드

저자

• Kyoung Ro KIM [ National Research Laboratory of Molecular Biotechnology and Dept. of Chemical Engineering, POSTECH, Pohang, 790-784. ]
• Dooyup JUNG [ National Research Laboratory of Molecular Biotechnology and Dept. of Chemical Engineering, POSTECH, Pohang, 790-784. ]
• Eun Hwa LEE [ National Research Laboratory of Molecular Biotechnology and Dept. of Chemical Engineering, POSTECH, Pohang, 790-784. ]
• Yoo Seong CHOI [ National Research Laboratory of Molecular Biotechnology and Dept. of Chemical Engineering, POSTECH, Pohang, 790-784. ]
• Hyung Joon CHA [ National Research Laboratory of Molecular Biotechnology and Dept. of Chemical Engineering, POSTECH, Pohang, 790-784. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576