In this report, we described a immunoassay method for CRP antigen detection by scFv conjugated AuNPs. Gold nanoparticles which are conjugated with anti-CRP scFvs. The functional scFv was expressed in cell-free protein synthesis system derived from an E. coli extract. And then the scFv was isolated by affinity-tag purification method. We have showed that the functional scFv was successfully prepared. And the scFvs could be used for direct detection its antigen in western blots when stained with anti-CRP scFvs. These results show that the cellfree system can produce a functional scFv and the anti-CRP scFv conjugated AuNPs could be used for detection of CRP antigen on the SPR surface. SPR measurements for detection of antigens were also possible with the use of this immobilized surface. The results suggested that the gold nanoparticle conjugation can be amplified the signal to SPR.
키워드
cell-free protein synthesisscFvAuNPs
저자
Ju-Young BYUN [ Biomonitering research center, Korea Research Institute of Bioscience and Biotechnology, Yuseong, Daejoen, Korea. ]
Dong-Myung KIM [ Department of Fine Chemical Engineering and Chemistry, Chungnam National University ]
Min-Gon KIM [ Biomonitering research center, Korea Research Institute of Bioscience and Biotechnology, Yuseong, Daejoen, Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
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