Earticle

초록

영어

Endocrine disruptor chemicals (EDCs) are exogeous substances which can disrupt the physiologic function of hormones in the endocrine system. Phage display technique can be used for selecting specific peptide for detection and elimination of these chemicals. However, the
traditional phage display methods, typically relying on batch adsorption/desorption procedures, provide only a single equilibrium stage for one round of selection, thus necessitating multiple rounds of repeated selection. Comparing to traditional method, phage display based on chromatographic biopanning using FPLC system provides a number of equilibrium stages, significantly increasing the screening efficiency. In our experiment, positive biopanning against Pb2+ as an endocrine disrupter chemical and negative biopanning to eliminate cross-binding peptides with non target metal ions such as Ni2+, Cu2+, Co2+, Fe3+ were performed. 17 Pb2+-affinity peptide binders have been identified and the relative affinity strength of these 17 peptide binders to Pb2+ is further determined through bead-based enzyme immunoassay (EIA) and number 3, 4, 1 and 5 peptide binders show higher affinity to Pb2+ than the others or the control peptide To the best of our knowledge, this study is the first to report Pb2+-specific peptide
binders, The selective Pb2+ cognitive peptide moieties are expected to provides many potential applications including biosensors and biosorbent for detection and removal of endocrine disruptors.

키워드

저자

• Hwijong KIM [ Dept. of Chemical Engineering, Sunhkyunkwan University ]
• Rui NIAN [ Dept. of Chemical Engineering, Sunhkyunkwan University ]
• Vo Thi Bich PHUONG [ Dept. of Chemical Engineering, Sunhkyunkwan University ]
• Woo Seok CHOE [ Dept. of Chemical Engineering, Sunhkyunkwan University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576