To identify novel genes that might be involved in tyrosine hydroxylase (TH) gene expression a preliminary microarray analysis of differential gene expression has been carried out on the olfactory bulbs (OBs) ipsilateral and contralateral to naris closure. Several developmental genes showing signifi cant differential regulation were identified based on microarrays analysis of OB mRNAs by Affymetrix GeneChip Mouse genome 430 2.0 arrays. These include Pbx1, pre-B-cell leukemia homeobox 1, Nkx2.1, also known as thyroid transcription factor 1, Evx1, the murine homologue of the even-skipped gene, and Prop1, a pituitary-specific paired-like homeodomain transcription factor. In addition, 36 ESTs for functionally uncharacterized genes were upregulated and 45 downregulated between 1.8 and 2.5 fold by naris closure. The expression of these genes and EST clones was confirmed by in situ hybridization and Northern blot techniques.
키워드
OBMicroarrayGene expression
저자
Hong Sung CHUN [ Dept. of Biotechnology, Chosun University, Gwangju, 501-759, Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동