Earticle

초록

영어

Single nucleotide polymorphism (SNP) is a DNA sequence variation occurring when a single nucleotide in the genome (or other shared sequence) differs between members of a species (or between paired chromosomes in an individual). Variations in the DNA sequences of humans can affect how humans develop diseases and respond to pathogens, chemicals, drugs, vaccines and other agents. There are many SNP detection methods such as taqman method, invader
method , MALDI-TOF method and Gene chip. But these methods need expensive equipments. For the cost-effective SNP detection, we used DNA duplex consisting of a reporter strand labeled with FAM or HEX and a sensor strand labeled with BHQ1. The sensor strand has a 6-nucleotide overhang that serves as a nucleation site. Depending on the SNP in a target oligonucleotide, the nucleation step is kinetically controlled. This leads to differential fluorescence changes, which can be used to identify the nucleotide at the SNP site. Because the FRETbased SNP detection method needs a conventional PCR cycler and a fluorometer, it enables SNP detection with a cost lower than the established procedures. AcknowledgementThis work was supported by BK21 program from the Korean Ministry of Education and Seoul R&BD Program NT080612, KU080657).

키워드

저자

• Soonmin LEE [ Dept. of Biotechnology, Yonsei University, Seoul. ]
• Jongshik SHIN [ Dept. of Biotechnology, Yonsei University, Seoul. ]

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간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576