Earticle

초록

영어

Consumption of spicy foods has been reported that it has thermogenic properties. Thus, those ingredients could be considered as potential agents which help preventing a positive energy balance and obesity. Specially, capsaicin (Cap) is the main capsaicinoids, which works pungent principle in red pepper, and is also utilized as a medicine. ated aboved In this study, twodimensional gel electrophoresis (2-DE) was carried out to identify the changes and/or differential expression of liver proteins in rats fed with highfat diet (HFD) in response to capsaicin treatments. In addition, immunoblot analysis of some liver proteins was examined for validation of proteomic analysis and suggestion of molecular action of capsaicin. Weight gain of rats
in the HFD+Cap group was decreased by 8% compare to HFD control group. We analyzed the differential expression patterns in rat’s liver using 2-DE in order to search for other proteins associated with thermogenesis and obesity. The proteomic analysis of liver sample demonstrated that about 120 spots were differentially expressed from a total 950 matched spots, of which 23 spots have been identified by peptide mass finger printing using MALDITOF
mass spectrometry. The protein levels of UCP2 and FAS in HFD+Cap group were decreased compared to HFD-Cap group, whereas those of p-AMPK, p-ACC and CPT-1 in HFD+Cap group were increased compared to HFD-Cap group. These data suggest that effect of capsaicin on energy expenditure and fatty acid oxidation in liver might be mediated through activating AMPK-ACC-malonyl-CoA metabolic signaling pathway.

키워드

저자

• Hee Sun HWANG [ Department of Biotechnology, Daegu University ]
• Jong Won YUN [ Department of Biotechnology, Daegu University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576