Transgenic plant cell culture is an attractive alternative for the production of recombinant proteins such as pharmaceutical proteins due to the absence of contaminants associated with mammalian or other culture systems. Plant cells are routinely maintained by periodic subculture that is a major drawback of the stable maintenance of high-producing cell lines1). To conform to existing regulatory requirements, plant cell cultures expressing biopharmaceuticals should be derived from a consistent and stable master stock. Cryopreservation would be an ideal method for retaining the original morphological forms and characteristics2). However, cryopreservation of plant cells has not been established yet because of the enormous difficulties for cell banking such as osmotic pretreatment, cryoprotection, freezing, thawing and regeneration. It is known that rice is resistant to anoxia, and sugar availability plays an important role3). In this study, transgenic suspension cells of Oryza sativa L. utilized as a host for producing recombinant human cytotoxic T lymphocyte-associated antigen 4-immunoglobulin (hCTLA4Ig) were preserved in anoxia condition for 2 months. Cell viability was stably maintained at 76% for 2 months in the media containing 174 mM glucose under anoxia condition, and the cells were successfully recovered. There were no significant differences in the cell growth and hCTLA4Ig production between non-preserved and 2-monthpreserved cells.
저자
Yong Suk Yang [ Department of Biological Engineering, Inha University ]
Ji-Yeon Han [ Department of Biological Engineering, Inha University ]
Su-Hwan Cheon [ Department of Biological Engineering, Inha University ]
Jun-Young Kwon [ Department of Biological Engineering, Inha University ]
Hye-Ran Lee [ Department of Biological Engineering, Inha University ]
Mi-Hee Yoo [ Department of Biological Engineering, Inha University ]
Ji-Won Choi [ Department of Biological Engineering, Inha University ]
Dong-Il Kim [ Department of Biological Engineering, Inha University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동