Increasing interest is now set in clinical applications of human mesenchymal stem cells (MSC). In order to explore there usages in medical applications, the ex vivo expansion of MSCs to sufficient cell numbers is necessary. Microcarrier cultures have been shown to allow extensive cell expansion of tissue engineering relevant cells, such as chondrocytes, while maintaining their phenotype. Our aim was to investigate the in vitro three-dimensional expansion of cord blooddrived mesenchymal stem cells using Hellex microcarrier. Hillex microcarrier showed the results for adherence with over 80% adherent cells after 3 h of incubation. We also cultivated the MSCs in 12 well plate to comparison with two dimensional monolayer cultivation. Results shown that the three dimensional expansion of MSC on microcarriers represents a beneficial alternative to the conventional two dimensional monolayer cultivation method.References 1. M. Du¨ rrschmid, K. Landauer, G. Simic, G. Blu¨ml and O. Doblhoff Dier Strategies for Microcarrier Based Animal Cell Bioprocesses BIOTECHNOLOGY AND BIOENGINEERING, VOL. 83, NO. 6, SEPTEMBER 20, 2003 681-686S. Tielens, H. Declercq, T. Gorski, E. Lippens, E. Schacht and M. Cornelissen Gelatin-Based Microcarriers as Embryonic Stem Cell Delivery System in Bone Tissue Engineering: An in-Vitro Study BIOMACROMOLECULES 2007, 8, 825-832.
저자
Qiu Yu JIANG [ Department of Bioscience&Biotechnology, sejong university ]
Jang Hyeon PARK [ BioR&D ]
Duk Jae OH [ Department of Bioscience&Biotechnology, sejong university ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동