Calcium is a critical parameter required for influencing the interaction of both the cell-cell and the cell-matrix. Extracellular calcium has strong potentiating effects on cell aggregation for diverse cell types in vitro. Cell aggregation during cell culture causes obstruction to nutrient and oxygen transfer, leading to cease of cell growth and eventually cell death. It has been reported that low calcium levels in serum-free media reduces aggregation of HEK293 cells and chondrocytes. In preceding studies, we observed the effect of high calcium concentrations on cell aggregation occurred with various recombinant Chinese Hamster Ovary-DG44 (rCHO-DG44) cell lines expressing different protein in suspension culture and found each rCHO-DG44 cell lines had different optimum calcium concentration on cell growth. In this study, we observed how the phenomenon of different aggregation characteristics by calcium levels with CHO-DG44 host cell line and various rCHO-DG44 cell lines recombination by same host cell line and expressing identical proteins but selected differently.
키워드
aggregationCHO-DG44 cell linesuspension culture
저자
Ui-Yeon KIM [ Dept. of Chemical Engineering, Hanyang University ]
Se-Jung KIM [ Dept. of Chemical Engineering, Hanyang University ]
Hong-Woo PARK [ Dept. of Chemical Engineering, Hanyang University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
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2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
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