Recombinant bovine trypsin was produced by genetically engineered transgenic rice cells using the sucrose starvation-inducible rice α-amylase 3D (RAmy3D) promoter. Batch culture was employed to investigate the effects of several alternative carbon sources on the trypsin production. In this study, succinic acid, malic acid and fumaric acid were used as substitutive carbon source to increase trypsin productivity. We have found that the productivity of recombinant trypsin increased 3.2- to 4.3-fold in medium containing an alternative carbon source compared with the control medium without carbon source. The accumulated recombinant trypsin in the medium containing fumaric acid reached 92.3 mg/L at day 7 after sugar starvation. Acknowledgments this work was supported by a grant from the Next Generation New Technology Development program of the Ministry of Commerce, Industry, and Energy
키워드
Alternative carbon sourceRecombinant trypsinRice cell suspension culture
저자
Hwa-Young YU [ Division of Biological Sciences, Chonbuk National University ]
Nan-Sun KIM [ Division of Biological Sciences, Chonbuk National University ]
Moon-Sik YANG [ Division of Biological Sciences, Chonbuk National University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동