Earticle

초록

영어

Plastic cell culture systems have a useful tool in research and biotechnlogy process, and a novel pneumatic bioteactor system (PBS) has been recently developed by PBS Biotech, Inc. In this study, to investigate the effects of agation speeds in 2L scale PBS, various rpm
settings (10, 20 and 30) were used for cultures of rCHO cells producing monoclonal antibody (MAb). The maximum cell density in PBS at 20 rpm reached 3.4 X 106 cells/ml, and final MAb concentration was 141 mg/L. Also, when culture of commercially available suspension cell line CHO-S (Invitrogen) was evaluated in PBS, the maximum cell density reached 10.5 X 106 cells/ml. similar result was obtained in stirred tank reactor (STR, 9.6 X 106 cells/ml), which was better than in Erlenmeyer flask (5.4 X 106 cells/ml). These results revealed that the novel PBS is a strong candidate with variety of advantages such as low cost and easy scale-up up to a few thousands liters in cell cultures compated to comventional STR. Reference. 1. Sing V. (1999) Disposable bioreactor for cell culture using wave-induced agitation. Cytotechnoloty. 30:149-158

키워드

저자

• Jae Choon KIM [ Dept, of Bioscience & Biotechnology, Sejong University ]
• Sung Geun LEE [ Dept, of Bioscience & Biotechnology, Sejong University ]
• Seong Min KIM [ Dept, of Bioscience & Biotechnology, Sejong University ]
• Brian LEE [ PBS Biotech, Inc.4023 Camino Ranchero, Suite #I, Camarillo, CA 93012, USA. ]
• Jang Hyeon PARK [ BIO R&D ]
• Duk Jae OH [ Dept, of Bioscience & Biotechnology, Sejong University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576