Stirred-tank bioreactor technologies have been used in animal cell culture systems. However, the high cost of stirred-tank bioreactor operation limits their availability (1). In contrast to stirred-tank bioreactor, disposable bag bioreactor reduces operation costs since it can eliminate the need for cleaning, sterilization, and validation. (2, 3). But the main problems confronted in using disposable bag bioreactor are agitation and oxygen mass transfer (4). The volumetric mass transfer coefficient (KLa) is a critical parameter for oxygen mass transfer. Adequate oxygen supply for animal cell culture is essential for the maximum cell proliferation and useful protein production. In this study, various prototype disposable bags were designed and its KLa was investigated. To increase KLa values, we used a twist shaking motion rather than tilting motion. In addition, a novel structure which can be installed at inside of disposable bag bioreactor had been developed for further increase of KLa. The novel structure showed enhanced KLa values in all types of disposable bag bioreactors and agitations.
키워드
Disposable bioreactorTwist shakingVolumteric mass transfer coefficient
저자
Sung-Hwan HONG [ Dept. of Biotechnology, School of Life Sciences and Biotechnology, Korea University ]
Juno JANG [ Dept. of Biotechnology, School of Life Sciences and Biotechnology, Korea University ]
Ik-Hwan KIM [ Dept. of Biotechnology, School of Life Sciences and Biotechnology, Korea University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동