Ji-Young LEE, Bok-Hwan CHUN, Yong Kwon LEE, Jang Han LEE, Won-Gi BANG, Tae Gon KIM, Jason AHN, Namhyun CHUNG
언어
영어(ENG)
URL
https://www.earticle.net/Article/A115262
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원문정보
초록
영어
A strong tendency is emerging to remove not only serum but also any product of animal origin from animal cell culture media during production for recombinant proteins. Plant protein hydrolysates have been considered promising substitutes for serum in mammalian cell cultures. The effects of individual plant protein hydrolysates on cell proliferation have been studied, but no attempt has been made to study the effects of mixtures of fractionated plant protein hydrolysates. We selected a few growth promoting protein hydrolysates (Broadbean, Soy F, and Soy P) and made mixture compositions with those hydrolysates, which considerably improved cell growth and viability compared with those seen in media containing each protein hydrolysate. We also compared the effects of individual fractionated protein hydrolysates with those of mixture composition with fractionated hydrolysates, and found that some of the mixture compositions containing a small fraction of a high–molecular-weight hydrolysate supported cell longevity greater than that seen in the control media containing unfractionated hydrolysates. We conclude that cell growth and viability may be improved in media containing a mixture of unfractionated or fractionated plant protein hydrolysates compared with media containing a single hydrolysate.
키워드
CHO cellsplant protein hydrolysatesmixture design
저자
Ji-Young LEE [ College of Life Science and Biotechnology, Korea University ]
Bok-Hwan CHUN [ College of Life Science and Biotechnology, Korea University, ]
Yong Kwon LEE [ Department of Food and Nutrition, Yuhan University ]
Jang Han LEE [ College of Life Science and Biotechnology, Korea University ]
Won-Gi BANG [ College of Life Science and Biotechnology, Korea University ]
Jason AHN [ Millipore Korea, Gangnam-gu, Seoul. ]
Tae Gon KIM [ Millipore Korea, Gangnam-gu, Seoul. 3Department of Food Sceince and Biotechnology, Kyungwon University ]
Namhyun CHUNG [ College of Life Science and Biotechnology, Korea University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동