Eun-Sun JEUNG, Yun-Ji SHIN, Hyo Boon KIM, Young-Eun YOO, Nan-Sun KIM, Hong-Soo DOO, Moon-Sik YANG, Tae-Ho KWON
언어
영어(ENG)
URL
https://www.earticle.net/Article/A115261
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
영어
Bovine trypsin is a pancreatic serine protease with substrate specificity based upon positively charged lysine and arginine side chains. It is derived from a 34kDa inactive precursor zymogen, trypsinogen, after enzymatic removal of an N-terminal 6-amino acid leader sequence resulting in the 24kDa trypsin molecule. It is widely used for commercial purposes to digest or process other proteins. Plant production system of recombinant proteins offers a way to replace animal-derived proteins with a safe and economical alternative. Recombinant bovine trypsin was produced in transgenic rice cell suspension culture with the rice amylase 3D promoter. It was purified from transgenic rice cell suspension culture by hydrophobic interaction chromatography and size exclusion chromatography. The enzyme activity of purified recombinant bovine trypsin was generally characterized by zymogram. The results demonstrate the use of recombinant bovine trypsin derived from plant cell suspension culture as protease.This work was supported by the grant from Korean Ministry of Knowledge Economy.
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동