The N-glycans of human granulocyte-macrophage colony-stimulating factor produced in genetically engineered rice cell suspension culture were analyzed. In previous study, immunoblot assay detection of secreted hGM-CSF revealed the presence of multiple glycoforms with molecular weights higher than observed for E.coli-derived hGM-CSF. In this study, N-glycan patterns of total secreted proteins were analyzed and then N-glycan patterns of secreted hGM-CSF were examined using HPLC and MALDI-TOF mass spectrometry. From the rice-derived hGM-CSF, seven types of N-glycns were MMXF3 (30.96%), GnMXF3 (44.76%), GnMX (1.84%), GnGnXF3 (8.73%), AF4MXF3 (4.76%), AGnXF3 (7.20%) and AF4GnXF3 (1.75%). The ratios of paucimannosidic-type (MMXF3) and complex-type glycoforms were 30%, 70%, respectively and nearly 20% of complex N-glycans showed Lewis a structure (This work was supported by the grant from Korean Ministry of Knowledge Economy)..
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동