Eun-Sun JEUNG, Yun-Ji SHIN, Hyo Boon KIM, Min-Woo NAM, Hong-Soo DOO, Tae-Ho KWON, Moon-Sik YANG
언어
영어(ENG)
URL
https://www.earticle.net/Article/A115258
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
영어
Factor X, a vitamin K-dependent blood coagulation glycoprotein, is the precursor for factor Xa, the enzyme component of the prothrombinase complex. The factor X is cleaved during coagulation by several different protease including the intrinsic Xase complex, the factor Xactivating enzyme from Russell's viper venom(RVV) and trypsin, and also by extrinsic pathway to give an active enzyme factor Xa. Factor Xa is often used to remove fusion tags from expressed proteins. Factor Xa cleaves after the arginine residue in its preferred cleavage site Ile- (Glu or Asp)-Gly-Arg. Human factor X was produced in transgenic rice cell suspension culture with the rice amylase 3D promoter. RAmy3D promoter is highly expressed by sugar starvation. The expression of human factor X as mRNA and protein in transgenic rice cell was detected Northern and Western blot analysis. The enzyme activity of RVV activated human factor X was generally characterized by using EGFP-hTNFa fusion protein as substrate, which contained a human factor Xa cleavage site between two moieties. These results demonstrate the use of human factor Xa derived from plant cell suspension culture as proteases (This work was supported by the grant from Korean Ministry of Knowledge Economy)..
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동