While there is clear evidence that granule and periglomerular cells of olfactory bulb originate in the anterior subventricular zone (SVZa), it is not known when they acquire the potential to become dopaminergic neurons. In this study, we investigated this issue utilizing a transgenic mouse with high level, tissue specific expression of lacZ under control of 9kb tyrosine hydroxylase (TH) promoter. Transgene expression was found in the neonatal, but not in the adult, rostral migratory stream (RMS) suggesting differential dopaminergic phenotypic regulation in developing and mature animals. Migrating cells were labeled using the lacZ gene expression kit and then substrate was delivered with the influx reagent and observed by 2-photon microscopy. Labeled cells were found in the RMS, glomerular and mitral cell layers between postnatal day 2 (P2) and P7. The adult RMS did not contain either THor β-gal-immunostained progenitor cells. These results suggest that dopaminergic phenotypic determination may finish in the developing neonatal stage.
키워드
Olfactory bulbMigrationRMS
저자
HongSung CHUN [ Dept. of Biotechnology, Chosun University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동