Earticle

초록

영어

Formate is generated from pyruvate and converted to carbon dioxide and hydrogen by formate hydrogen lyase (FHL). FHL enzyme consists of many proteins including two formate dehydrogenase H (FDH-H) and hydrogenase (Hyd3). This enzyme complex is responsible for
hydrogen production in E. coli cells during fermentation of organic carbons. In order to improve the activity of FHL in whole cell, a series of E. coli mutant strains were constructed using E. coli BW25113 as parent strain. First of all, hyaAB and hybBC, encoding hydrogenase 1 and hydrogenase 2, respectively, which catalyze hydrogen uptake reactions, were disrupted. Then, ldhA, encoding lactate dehydrogenase and frdAB encoding fumarate reductase were deleted to construct the resulting strain, SH5 (hyaAB, hybBC, ldhA and frdAB). This SH5 strain was further manipulated to construct SH5-5 strain which has two more modifications from SH5: (i) Deletion of IscR, an iron-sulfur cluster repressor which reduces the transcription of iron-sulfur cluster
operon, and (ii) overexpression of fhlA, a FHL transcriptional activator. The SH5-5 (ΔiscR, and up-regulated fhlA) showed an increased specific activity of 2.6 μmol H2 mg-1 min-1. In omparison, the parental E. coli BW25113 showed the specific activity of 1.2 μmol H2 mg-1 min-1 and SH5 showed1.4 μmol H2 mg-1 min-1, respectively.

키워드

저자

• Younga JANG [ Dept. of Chemical and Biochemical Eng, Pusan National University ]
• Eunhee SEOL [ Dept. of Chemical and Biochemical Eng, Pusan National University ]
• Seohyung KIM [ Dept. of Chemical and Biochemical Eng, Pusan National University ]
• Sunghoon PARK [ Dept. of Chemical and Biochemical Eng, Pusan National University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576