Earticle

초록

영어

Microalga Chlorella vulgaris C-169 was studied to optimize its growth and lipid synthesis in flask cultures. Important parameters investigated include kinds of medium (N8 and Bristol), nitrogen source (nitrate 1-20 mM, ammonium 1 – 30 mM), phosphate buffer (1-15 mM), Fe (0-0.3 mM), and pH (5.5-8.5). Cell density, pH and intracellular concentrations of protein, carbohydrate, chlorophyll, and fatty acids were monitored during cultivation. Overall nitrate was a better source
than ammonium for cell growth and lipid synthesis. When phosphate buffer concentration increased, the final cell density increased although intracellular fatty acids content did not change. Optimal Fe concentration was 0.01 mM. Under tested pH range, optimal pH for cell growth was 6.5, but highest fatty acids accumulation was observed at 7.5. The maximal cell density and intracellular fatty acids content obtained under partially optimized conditions were 2.0 g/L and 320 mg/g cell, respectively. Major fatty acids were palmitic acid (15% of total fatty
acids), stearic acid (8%), oleic acid (50%), linoleic acid (14%), and α- linolenic acid (13%). Other optimization experiments are under progress.

키워드

저자

• Kyoung Ae SEO [ Bioenergy Research Center, Korea Institute of Energy Research ]
• Mi SUNG [ Bioenergy Research Center, Korea Institute of Energy Research ]
• Soon Chul PARK [ Bioenergy Research Center, Korea Institute of Energy Research ]
• Joon Pyo LEE [ Bioenergy Research Center, Korea Institute of Energy Research ]
• You-Kwan OH [ Bioenergy Research Center, Korea Institute of Energy Research ]

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간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576