Min OK CHO, Sun-Mi LEE, Youngsoon UM, Byoung-In SANG
언어
영어(ENG)
URL
https://www.earticle.net/Article/A115211
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
영어
Biobutanol has many characteristics that make it a better biofuel than bioethanol, now used in the formulation of gasohol. Despite the remarkable advantages of butanol as a fuel, biobutanol production has several limitations, including degeneration. Solventogenic clostridia lose their solvent formation capabilities during repeated sub-culturing of batch cultures or during a continuous fermentation. This phenomenon is known as 'degeneration'. The degeneration is caused by the loss of the pSOL1 plasmid that carries essential genes involved in solvent production. Therefore, a method is needed to detect and quantify the pSOL1 plasmid in Clostridium acetobutylicum ATCC 824. In this study, we designed two specific primer and probe sets for multiplex real-time qPCR detection of C.acetobutylicum ATCC 824 (the aceto set) and pSOL1 plasmid possessing C.acetobutylicum ATCC 824 (the DGS set). Specific primer and probe sets were designed on the basis of the 16S rDNA sequence and pSOL1 sequence. To confirm the effectiveness of the technique, the multiplex real-time qPCR results were compared with those obtained by plating. In conclusion, using multiplex real-time qPCR, degenerated Clostridium acetobutylicum was successfully detected and quantified.
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동