Mussel adhesive proteins (MAPs) have a potential in diverse biotechnological and medical applications. However, natural extraction is inefficient for large-scale use and mass-production of recombinant MAPs has limited or failed according to foot protein (fp) types in several expression systems. Previously, to improve the production yield of recombinant MAPs, we designed new types of MAP hybrids (fp-151 and fp-353) and tried to express in Escherichia coli. Due to their successful expression with superior properties such as high production yield, simple purification, and strong adhesive ability, they have been investigated in many practical uses. In the present work, we designed and constructed various MAP hybrids that are composed of fp-1 (6- or 12-repeated decapeptides), fp- 3, and fp-5 to investigate combination effect of each fp types and size effect of each MAP hybrid on adhesive ability, water resistance, and other properties in practical applications. We tried to express new MAP hybrids and optimized expression levels under various culture conditions.
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
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