Earticle

초록

영어

One of the putative NRPS (Nonribosomal Peptide Synthetase) gene clusters of Streptomyces peucetius ATCC 27952 is selected and its domain architecture is predicted as C-A-T-C-A-T-E-C-A-T-C-A-T-C by homology search within NRPS database. The product of this gene cluster is predicted as a siderophore that is ferric-specific ligand to scavenge and transport iron into the cells. In previous study, isolated peptides were confirmed to be hydroxamate type
siderophore by Universal CAS assay, Csaky test. Using a gene knockout/comparative metabolic profiling, it is confirmed that this peptide is synthesized by NRPS encoded by sp970 gene cluster. As a result of the deletion of sp970 gene cluster, it is confirmed that S. peucetius mutant does not produce the peptide. The mass spectrum of this peptide shows 4 series of molecular ions (m/z 645, 659, 673, 687). Through tandem mass spectrometric analysis, the structure of this peptide can be proposed as cyclic form which consists of four amino acids, N-acetyl-formyl-hydroxy Ornithine, Arginine, hydroxyl Ornithine, formyl-hydroxy Ornithine, and neutral modification. In future work, it plans to elucidate the exact structure of the peptide by using GC-MS analysis for characterization of this nonribosomal peptide.This present study is the discovery
of a novel nonribosomal peptide product from Streptomyces peucetiusby genome mining and mass spectrometry.

키워드

저자

• Hae-Min PARK [ School of Chemical and Biological Engineering, Seoul National University ]
• Byung-Gee KIM [ School of Chemical and Biological Engineering, Seoul National University ]
• Dong Sook JANG [ School of Chemical and Biological Engineering, Seoul National University ]
• Hwang-Soo JOO [ School of Chemical and Biological Engineering, Seoul National University ]
• Pyoung Il KIM [ School of Chemical and Biological Engineering, Seoul National University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576